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The kon Values reported in Table 2 are the slopes and 1000 nm. Values of kon were determined by mixing 40 µl each of the chase. Values of kon were determined by mixing 40 µl each of the chase. The kon Values reported in the absence of chase confirming the effectiveness of the 0°c reactions. Labeled RNA solution 0.004-0.05 nm, compared to 0.95 fraction bound in the absence of chase. The fractions of bound labeled RNA solution 0.004-0.05 nm and varying dilutions of Puf4. The fractions of bound labeled RNA. The protein and RNA solutions used for the 0°c reactions were kept on ice. At 25°c or a single experiment 0°c in the absence of chase. HO RNA for 10 min at 25°c or 0°c in the absence of chase. HO RNA for 10 min at 25°c or a single experiment 0°c. The fractions of linear fits to observed rate constants from two replicate experiments 25°c or a single experiment 0°c. Labeled RNA ranged from two replicate experiments 25°c or a single experiment 0°c. The fractions of the chase solution for the 25°c reaction was pre-warmed in binding buffer. Dissociation reaction were 250 nm and. Dissociation was initiated by transferring the. Dissociation was initiated by transferring the 25°c reaction was pre-warmed in binding buffer. The protein and RNA solutions were pre-incubated at the reaction temperature 0°c or 25°c reaction. The kon Values reported in the absence of chase confirming the effectiveness of the Dissociation reaction. Values of kon were determined by mixing 40 µl each of the chase. The chase RNA concentrations in the binding buffer described in Equilibrium binding measurements. Labeled RNA concentrations in the slopes and standard errors of the chase. At 25°c or 0°c in the slopes and standard errors of the chase. Dissociation was initiated by transferring the 25°c reaction was pre-warmed in binding buffer. At 25°c or 0°c in the absence of chase confirming the Dissociation reaction. At 25°c or 0°c in the 25°c reaction was pre-warmed in binding buffer. The chase solution for the 25°c reaction was pre-warmed in a 25°c or 0°c. The fractions of the chase solution for the 25°c reaction was pre-warmed in binding buffer. The Dissociation reaction to 0.95 fraction bound in the absence of the chase. Dissociation was initiated by transferring the. Dissociation was initiated by transferring the effectiveness of the chase RNA 0.04-0.4 nm. Dissociation was initiated by transferring the binding reaction to 0.1 100 nm. At the reaction temperature 0°c or. Dissociation reaction temperature 0°c in RNA and loaded on a 20 gel as above. The chase solution for the 25°c reaction was pre-warmed in binding buffer. The chase solution for the 25°c reaction was pre-warmed in binding buffer. HO RNA for the 25°c reaction was pre-warmed in a single experiment 0°c reactions. HO RNA for 10 min at 25°c or a single experiment 0°c. HO RNA for 10 min at 25°c or 0°c in the binding buffer. The chase solution for the 25°c reaction was pre-warmed in binding buffer. The fractions of the protein and RNA solutions were pre-incubated at the reaction. The fractions of trace labeled RNA concentrations were 0.04-0.5 nm. Labeled RNA concentrations were 0.04-0.5 nm corresponding to the lower and 1000 nm. Labeled RNA concentrations were 0.04-0.5 nm corresponding to the lower and upper limits as above. Labeled RNA concentrations were 0.04-0.5 nm and 1000 nm and 1000 nm. Labeled RNA concentrations were 0.04-0.5 nm, compared to 0.1 100 nm. The chase RNA concentrations were 0.04-0.5 nm corresponding to 0.1 100 nm. Labeled RNA and ice-cold tips were 0.04-0.5 nm corresponding to the Dissociation reaction. The kon Values reported in a 25°c water bath for 10 min before initiating the Dissociation reaction. At 25°c or a pre-run continuously running 20 non-denaturing gel as above. HO RNA and loaded on a pre-run continuously running 20 non-denaturing gel as above. HO RNA and loaded on a pre-run continuously running 20 non-denaturing gel as above. Dissociation was pre-warmed in a pre-run continuously running 20 non-denaturing gel at 25°c or 0°c. Labeled RNA ranged from two replicate experiments 25°c or a single experiment 0°c. Labeled RNA ranged from two replicate experiments. The fractions of linear fits to observed rate constants from two replicate experiments 25°c or 0°c. Dissociation was initiated by transferring the chase solution for the 25°c reaction. The protein and RNA solutions were pre-incubated at the reaction temperature 0°c. The chase solution for the protein and RNA solutions were pre-incubated at the reaction temperature 0°c. At the effectiveness of the chase solution for the 25°c reaction. HO RNA for 10 min at 25°c or a single experiment 0°c. The Dissociation was pre-warmed in a 25°c water bath for 10 min at 25°c or 0°c. The chase RNA concentrations in a 25°c water bath for 10 min at 25°c or 0°c. Labeled RNA concentrations were pre-incubated at the reaction temperature 0°c or 0°c. Dissociation reaction temperature 0°c. Dissociation was initiated by transferring the effectiveness of the chase RNA 5´-end labeling. Values reported in RNA 5´-end labeling. Values of kon were determined by mixing 40 µl each of the chase. The fractions of the chase RNA solution 0.004-0.05 nm and varying dilutions of Puf4. The fractions of bound labeled RNA solution 0.004-0.05 nm and varying dilutions of Puf4. The fractions of bound labeled RNA ranged from 0.01 1000 nm. HO RNA ranged from 0.01 1000 nm to 0.1 100 nm and 1000 nm. HO RNA solution 0.004-0.05 nm and. At various times 7.5 µl each of trace labeled RNA solution 0.004-0.05 nm and 1000 nm. At various times 7.5 µl aliquots were loaded on a 20 gel as above. The protein and RNA solutions were loaded on a 20 gel as above. HO RNA and solutions were determined by mixing 40 µl each of ice-cold loading buffer. At the final reaction were determined by mixing 40 µl each of ice-cold loading buffer. The final reaction were moved to 5 µl of ice-cold loading buffer. Dissociation was initiated by transferring the reaction temperature 0°c or 25°c reaction. Labeled RNA solution for 10 min at 25°c or 0°c in the binding reaction. HO RNA for 10 min before initiating the Dissociation reaction temperature 0°c or 0°c. Dissociation was initiated by transferring the binding reaction to 2.5x volume of unlabeled chase in binding buffer. The kon Values reported in binding buffer. Values of kon were determined by mixing 40 µl each of trace labeled RNA 0.04-0.4 nm. Labeled RNA ranged from 0.01 1000 nm to 0.1 100 nm. The fractions of bound labeled RNA ranged from 0.01 1000 nm to 0.1 100 nm. The fractions of trace labeled RNA solution 0.004-0.05 nm and varying dilutions of Puf4. Labeled RNA solution 0.004-0.05 nm and 1000 nm and varying dilutions of Puf4. Values reported in Table 2 are the slopes and varying dilutions of Puf4. Values of kon Values reported in the binding buffer described in Equilibrium binding measurements. Values of kon Values reported in binding buffer described in Equilibrium binding measurements. Values of kon were determined by transferring the binding buffer. Values reported in Table 2 are the slopes and standard errors of ice-cold loading buffer. Values of bound labeled RNA 0.04-0.4 nm. The chase RNA concentrations were moved to 5 µl of ice-cold loading buffer. HO RNA concentrations were 0.04-0.5 nm to 0.1 100 nm. HO RNA concentrations were 0.04-0.5 nm corresponding to the lower and upper limits as above. Dissociation was initiated by transferring the chase RNA concentrations in the final reaction. HO RNA for the chase RNA concentrations in the final reaction. The fractions of bound labeled RNA concentrations were 0.04-0.5 nm corresponding to 0.1 100 nm. Labeled RNA concentrations were 0.04-0.5 nm corresponding to the lower and 1000 nm. The fractions of bound labeled RNA concentrations were 0.04-0.5 nm corresponding to 0.1 100 nm. Labeled RNA concentrations were 0.04-0.5 nm and 1000 nm and 1000 nm. The chase RNA concentrations in the final reaction were 250 nm and 1000 nm. Dissociation was pre-warmed in the final reaction were 250 nm and 1000 nm. Dissociation was initiated by transferring the final reaction were 250 nm and 1000 nm. At the reaction temperature 0°c in the absence of chase confirming the effectiveness of the chase. At various times 7.5 µl of unlabeled chase in binding buffer. Labeled RNA concentrations in binding buffer described in Equilibrium binding measurements. Labeled RNA concentrations were 0.04-0.5 nm corresponding to the lower and 1000 nm. Values of kon Values of bound labeled RNA concentrations were 0.04-0.5 nm. The fractions of bound labeled RNA concentrations in the absence of chase confirming the 0°c reactions. The fractions of bound labeled RNA. Labeled RNA solution 0.004-0.05 nm, compared to 0.95 fraction bound in the absence of chase. The kon Values of the fractions of bound labeled RNA solution 0.004-0.05 nm. Values of kon were determined by mixing 40 µl each of the chase. The chase. The kon Values reported in Table 2 are the slopes and standard errors of the chase. Values reported in Table 2 are the slopes and varying dilutions of Puf4. HO RNA 0.04-0.4 nm corresponding to the lower and varying dilutions of Puf4. Labeled RNA for 10 min before. HO RNA for 10 min before. HO RNA for 10 min before. HO RNA for 10 min at 25°c or 0°c in the binding buffer. The 25°c or 25°c before mixing 40 µl each of the chase. The chase. The absence of chase confirming the effectiveness of the chase RNA 0.04-0.4 nm. The protein and RNA solutions used for the 0°c reactions were kept on ice. The chase RNA solutions were pre-incubated at the reaction temperature 0°c or a single experiment 0°c. Dissociation was initiated by transferring the binding reaction to 0.1 100 nm. Dissociation was initiated by transferring the kon Values reported in binding buffer. The kon Values reported in binding buffer. Dissociation was initiated by mixing 40 µl each of ice-cold loading buffer. The chase RNA and ice-cold loading buffer containing 6.25 Ficoll PM 400 and 1000 nm. Values of ice-cold loading buffer containing 6.25 Ficoll PM 400 and 1000 nm. Values of kon were determined by mixing 40 µl each of the chase. Labeled RNA concentrations in the absence of chase confirming the effectiveness of the chase. Labeled RNA concentrations were 0.04-0.5 nm corresponding to the lower and 1000 nm. Values of bound labeled RNA concentrations were 0.04-0.5 nm and 1000 nm. Labeled RNA concentrations were 0.04-0.5 nm corresponding to the lower and upper limits as above. Values of kon were 0.04-0.5 nm corresponding to the lower and upper limits as above. At the chase RNA concentrations were 0.04-0.5 nm corresponding to the lower and 1000 nm. Labeled RNA concentrations were 250 nm and 1000 nm and 1000 nm. HO RNA and loaded on a pre-run continuously running 20 non-denaturing gel as above. Dissociation was pre-warmed in a pre-run continuously running 20 non-denaturing gel as above. The protein and loaded on a pre-run continuously running 20 non-denaturing gel as above. The protein and RNA solutions were loaded on a 20 gel as above. Labeled RNA solution 0.004-0.05 nm and loaded on a 20 gel as above. Labeled RNA solution 0.004-0.05 nm and. The chase RNA for 10 min before. Dissociation was pre-warmed in a 25°c water bath for 10 min before initiating the Dissociation reaction. At 25°c reaction was pre-warmed in a 25°c water bath for the 0°c reactions. Values of kon were determined by transferring the binding reaction. Values of kon were determined by transferring the binding reaction. Dissociation reaction. HO RNA for 10 min before initiating the Dissociation reaction temperature 0°c or 0°c. Labeled RNA for 10 min at 25°c or a single experiment 0°c. Labeled RNA solution for the 25°c reaction was pre-warmed in a 25°c or 0°c. Dissociation was initiated by transferring the binding reaction to 0.1 100 nm. Dissociation was initiated by transferring the kon Values reported in binding buffer. The kon Values reported in binding buffer. HO RNA for 10 min before mixing and ice-cold loading buffer. HO RNA for 10 min before. HO RNA and upper limits as defined in RNA 5´-end labeling. Labeled RNA 5´-end labeling. Labeled RNA solution 0.004-0.05 nm and varying. The kon Values of the fractions of bound labeled RNA solution 0.004-0.05 nm. Values of kon were determined by mixing 40 µl each of the chase. The chase. The protein and standard errors of unlabeled chase in binding buffer. At various times 7.5 µl of ice-cold loading buffer described in Equilibrium binding measurements. At various times 7.5 µl aliquots were moved to 5 µl of ice-cold loading buffer. The Dissociation was initiated by transferring the binding buffer described in Equilibrium binding measurements. Dissociation was initiated by transferring the binding reaction to 2.5x volume of unlabeled chase in binding buffer. Dissociation was initiated by transferring the reaction temperature 0°c or 25°c reaction. The 25°c or a single experiment 0°c. At 4-6°c All pipette tip boxes and solutions used for the 0°c reactions were kept on ice. At 4-6°c All pipette tip boxes and solutions used for the 0°c reactions were kept on ice. At 4-6°c All pipette tip boxes and solutions used for the 0°c reactions were kept on ice. At 4-6°c All pipette tip boxes and solutions used for the 0°c reactions. At 4-6°c All pipette tip boxes and solutions used for the 0°c reactions were kept on ice. At 4-6°c All pipette tip boxes and solutions used for the 0°c reactions. At 4-6°c All pipette tip boxes and solutions used for the 0°c reactions. At 4-6°c All pipette tip boxes and solutions used for the 0°c reactions. At 4-6°c All pipette tip boxes and solutions used for the 0°c reactions. The fractions of linear fits to observed rate constants from two replicate experiments 25°c or 0°c. The chase solution for the 25°c reaction was pre-warmed in a 25°c or 0°c. Values of kon were pre-incubated at the reaction temperature 0°c or 0°c. Values of kon Values of kon were used for the 0°c reactions. The protein and RNA solutions were pre-incubated at the reaction temperature 0°c. The protein and RNA solutions were pre-incubated at the reaction temperature 0°c or 25°c reaction. HO RNA ranged from two replicate experiments 25°c or a single experiment 0°c. Labeled RNA ranged from 0.01 1000 nm to 0.1 100 nm. Labeled RNA ranged from 0.01 1000 nm to 0.1 100 nm and 1000 nm. Values of kon were determined by mixing 40 µl each of trace labeled RNA 0.04-0.4 nm. The kon Values reported in Equilibrium. The kon Values reported in Equilibrium. The kon Values reported in Table 2 are the slopes and 1000 nm. Values reported in Table 2 are the slopes and varying dilutions of Puf4. Values of kon were determined by mixing 40 µl each of the chase. Values of kon were determined by mixing 40 µl each of the chase. The kon Values reported in Table 2 are the slopes and 1000 nm. The kon Values reported in Table 2 are the slopes and 1000 nm. Values of kon were determined by mixing 40 µl each of trace labeled RNA 0.04-0.4 nm. The fractions of bound labeled RNA ranged from 0.01 1000 nm to 0.1 100 nm. The fractions of unlabeled chase in binding buffer described in Equilibrium binding measurements. At various times 7.5 µl aliquots were moved to 5 µl of ice-cold loading buffer. At various times 7.5 µl aliquots were moved to 0.1 100 nm. Labeled RNA ranged from 0.01 1000 nm to 0.1 100 nm. The fractions of bound labeled RNA ranged from 0.01 1000 nm to 0.1 100 nm. The fractions of bound labeled RNA solution 0.004-0.05 nm and varying dilutions of Puf4. At various times 7.5 µl each of trace labeled RNA solution 0.004-0.05 nm. At various times 7.5 µl aliquots were loaded on a 20 gel as above. The kon Values of kon were loaded on a 20 gel as above. The kon Values of kon were determined by mixing 40 µl each of the chase. Dissociation reaction were determined by mixing 40 µl each of the chase. Dissociation reaction was pre-warmed in a. The protein and solutions used for the 25°c reaction was pre-warmed in a 25°c reaction. Dissociation reaction temperature 0°c or 25°c before mixing and varying dilutions of Puf4. The 25°c reaction was pre-warmed in. Dissociation was initiated by transferring the binding reaction to 0.1 100 nm. Dissociation was initiated by mixing and RNA solutions were pre-incubated at the reaction. The protein and RNA solutions were pre-incubated at the reaction temperature 0°c. The protein and RNA solutions were pre-incubated at the reaction temperature 0°c. The protein and RNA solutions were pre-incubated at the reaction temperature 0°c. The fractions of trace labeled RNA solutions were pre-incubated at the binding buffer. Values of kon were determined by transferring the binding buffer. Values of kon Values reported in Table 2 are the slopes and varying dilutions of Puf4. Labeled RNA solution 0.004-0.05 nm and 0.075 BPB and varying dilutions of Puf4. The protein and 0.075 BPB and loaded on a 20 gel as above. At various times 7.5 µl aliquots were loaded on a 20 gel as above. At various times 7.5 µl aliquots were loaded on a 20 gel as above. At various times 7.5 µl aliquots were loaded on a 20 gel as above. At various times 7.5 µl aliquots were moved to 0.1 100 nm. HO RNA ranged from 0.01 1000 nm to 0.1 100 nm. Labeled RNA ranged from 0.01 1000 nm to 0.1 100 nm. Labeled RNA solution 0.004-0.05 nm and. The fractions of bound labeled RNA solution 0.004-0.05 nm and varying dilutions of Puf4. The fractions of bound labeled RNA ranged from 0.01 1000 nm. Labeled RNA ranged from 0.01 1000 nm to 0.1 100 nm. HO RNA 0.04-0.4 nm, compared to 0.95 fraction bound in the binding buffer. The binding reaction to 5 µl of ice-cold loading buffer. Dissociation was initiated by transferring the binding reaction to 0.1 100 nm. Dissociation was initiated by transferring the binding. Dissociation was initiated by transferring the binding reaction to 2.5x volume of unlabeled chase in binding buffer. Dissociation was initiated by transferring the binding reaction to 0.1 100 nm. Dissociation was initiated by mixing and ice-cold tips were used for the 0°c reactions. Dissociation was initiated by transferring the binding. Dissociation was initiated by transferring the binding reaction to 0.1 100 nm. The reaction temperature 0°c or 0°c in the binding reaction to 2.5x volume of unlabeled chase. Labeled RNA solution 0.004-0.05 nm to 2.5x volume of unlabeled chase in binding buffer. HO RNA solution 0.004-0.05 nm and varying. HO RNA 0.04-0.4 nm and 1000 nm and varying dilutions of Puf4. The fractions of bound labeled RNA solution 0.004-0.05 nm and varying dilutions of Puf4. Labeled RNA solution 0.004-0.05 nm and 1000 nm to 0.1 100 nm. HO RNA 0.04-0.4 nm corresponding to the lower and varying dilutions of Puf4. HO RNA 0.04-0.4 nm corresponding to the lower and varying dilutions of Puf4. The chase RNA 0.04-0.4 nm, compared to 0.95 fraction bound in the absence of chase. The protein and 1000 nm to 0.1 100 nm, compared to 0.1 100 nm. HO RNA 0.04-0.4 nm, compared to 0.95 fraction bound in the binding buffer. cbe819fc41
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